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Overexpression of a SNARE protein AtBS14b alters BR response in Arabidopsis

Zhong Xin Zhu1, Hong Bo Ye3, Yuan Hu Xuan2* and Da Nian Yao1*

Author Affiliations

1 Agricultural College, Anhui Agricultural University, Changjiangxi Road 130, Hefei 230036, Anhui, China

2 College of Pharmaceutical Sciences, Wenzhou Medical University, Xueyuanxi Road 82, Wenzhou 325035, Zhejiang, China

3 Department of Biology, Stanford University, N. Service Road, Stanford 94305-5020, CA, USA

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Botanical Studies 2014, 55:55  doi:10.1186/s40529-014-0055-5

Published: 12 July 2014

Abstract

Background

N-ethyl-maleimide sensitive factor adaptor protein receptor (SNAREs) domain-containing proteins were known as key players in vesicle-associated membrane fusion. Genetic screening has revealed the function of SNAREs in different aspects of plant biology, but the role of many SNAREs are still unknown. In this study, we have characterized the role of Arabidopsis Qc-SNARE protein AtBS14b in brassinosteroids (BRs) signaling pathway.

Results

AtBS14b overexpression (AtBS14b ox) plants exhibited short hypocotyl and petioles lengths as well as insensitivity to exogenously supplied BR, while AtBS14b mutants did not show any visible BR-dependent morphological differences. BR biosynthesis enzyme BR6OX2 expression was slightly lower in AtBS14b ox than in wild type plants. Further BR-mediated repression of BR6OX2, CPD and DWF4 was inhibited in AtBS14b ox plants. AtBS14b-mCherry fusion protein localized in vesicular compartments surrounding plasma membrane in N. benthamiana leaves. In addition, isolation of AtBS14b-interacting BR signaling protein, which localized in plasma membrane, showed that AtBS14b directly interacted with membrane steroid binding protein 1 (MSBP1), but did not interact with BAK1 or BRI1.

Conclusion

These data suggested that Qc-SNARE protein AtBS14b is the first SNARE protein identified that interacts with MSBP1, and the overexpression of AtBS14b modulates BR response in Arabidopsis.

Keywords:
SNARE; Trafficking; BR; MSBP1; Arabidopsis